Gene Expression Analysis by Real-Time PCR:
PCR (Polymerase Chain Reaction) is a powerful molecular biology technique used for gene expression analysis. It allows for the amplification of specific DNA sequences, enabling researchers to study the expression levels of target genes.
SYBR Green is a fluorescent dye commonly used in quantitative PCR (qPCR) for gene expression analysis. It is a DNA-binding dye that fluoresces when bound to double-stranded DNA. SYBR Green-based qPCR is a widely adopted method for real-time monitoring of DNA amplification during the PCR reaction. Here’s an overview of the PCR gene expression analysis using SYBR Green.
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